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- P-ISSN2287-8327
- E-ISSN2288-1220
- SCOPUS, KCI
ISSN : 2287-8327
Two-year field monitoring shows little evidence that transgenic potato containing ABF3 significantly alters its rhizosphere microbial community structure
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Abstract
Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional “Jopoong” plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.
- keywords
- Biosafety, Community composition, Drought tolerance, T-RFLP, Water deficit
Reference
Abdeen, A., Schnell, J., & Miki, B. (2010). Transcriptome analysis reveals absence of unintended effects in drought tolerant transgenic plants overexpressing the transcription factor ABF3. BMC Genomics, 2, 69–89.
Bapiri, A., Bååth, E., & Rousk, J. (2010). Drying–rewetting cycles affect fungal and bacterial growth differently in an arable soil. Microbial Ecology, 60, 419–428.
Barnard, R. L., Osborne, C. A., & Firestone, M. K. (2013). Responses of soil bacterial and fungal communities to extreme desiccation and rewetting. The ISME Journal, 7, 1–13.
Chen, D., Zhou, L., Wu, J., Hsu, J., Lin, Y., & Fu, S. (2012). Tree girdling affects the soil microbial community by modifying resource availability in two subtropical plantations. Applied Soil Ecology, 53, 108–115.
Choi, H., Hong, J., Kang, J., & Kim, S. Y. (2000). ABFs, a family of ABA-responsive element binding factors. Journal of Biological Chemistry, 21, 1723–1730.
Chun, Y. J., Kim, H.-J., Park, K. W., Jeong, S.-C., Lee, B., Back, K., Kim, H. M., & Kim,C.-G. (2012). Two-year field study shows little evidence that PPO-transgenic rice affects the structure of soil microbial communities. Biology and Fertility of Soils, 48, 453–461.
Clarke, K. R., & Gorley, R. N. (2006). Primer v6: user manual/tutorial. Plymouth, UK:PRIMER-E.
Domagalska, M. A., Sarnowska, E., Nagy, F., & Davis, S. J. (2010). Genetic analyses of interactions among gibberellin, abscisic acid, and brassinosteroids in the control of flowering time in Arabidopsis thaliana. PLoS ONE, 5(11), e14012.
Gürtler, V., & Stanisich, V. A. (1996). New approaches to typing and identification of bacteria using the 16S–23S rDNA spacer region. Microbiology, 142, 3–16.
Hawkes, C. V., Kivlin, S. N., Rocca, J. D., Huguet, V., Thomsen, M. A., & Suttle, K. B.(2011). Fungal community responses to precipitation. Global Change Biology, 17, 1637–1645.
Heuer, H., Kroppenstedt, R. H., Lottman, J., Berg, G., & Smalla, K. (2002). Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors. Applied and Environmental Microbiology, 68, 1325–1335.
Kang, J., Choi, H., Im, M., & Kim, S. Y. (2002). Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell, 14, 343–357.
Kim, S. Y. (2007). Development of drought-tolerant rice varieties. Report of the Crop Functional Genomics Centre. Korea.
Kim, J. B., Kang, J. Y., & Kim, S. Y. (2004). Over-expression of a transcription factor regulating ABA responsive gene expression confers multiple stress tolerance. Plant Biotechnology Journal, 2, 459–466.
Kim, C.-G., Kim, D. Y., Moon, Y. S., Kim, H. J., Kim, D. I., Chun, Y. J., Park, K. W., Jeong, S.C., Kim, S. Y., & Kim, H. M. (2010). Persistence of genetically modified potatoes in the field. Journal of Plant Biology, 53, 395–399.
Korea Meteorological Administration (2010). Climate data: http://www.kma.go.kr/weather/climate/past_cal.jsp. Accessed 11 Jan 2017.
Landesman, W. J., & Dighton, J. (2010). Response of soil microbial communities and the production of plant-available nitrogen to a two-year rainfall manipulation in the New Jersey Pinelands. Soil Biology & Biochemistry, 42, 751–1758.
Leckie, S. E., Prescott, C. E., Grayston, S. J., Neufeld, J. D., & Mohn, W. W. (2004). Characteriaztion of humus microbial communities in adjacent forest types that differ in nitrogen availability. Microbial Ecology, 48, 29–40.
Lord, N. S., Kaplan, C. W., Shank, P., Kitts, C. L., & Elrod, S. L. (2002). Assessment of fungal diversity using terminal restriction fragment (TRF) pattern analysis:comparison of 18S and ITS ribosomal regions. FEMS Microbiology Ecology, 42, 327–337.
Mkhabela, M., Bullock, P., Gervais, M., Finlay, G., & Sapirstein, H. (2010). Assessing indicators of agricultural drought impacts on spring wheat yield and quality on the Canadian prairies. Agricultural and Forest Meteorology, 150, 399–410.
Odell, J. T., Nagy, F., & Chua, N.-H. (1985). Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature, 313, 810–812.
Pietri, J. C. A., & Brookes, P. C. (2009). Substrate inputs and pH as factors controlling microbial biomass, activity and community structure in an arable soil. Soil Biology & Biochemistry, 41, 1396–1405.
R_Core_Team. (2013). R: a language and environment for statistical computing. Vienna, Austria: R foundation for statistical computing. http://www.R-project. org/. ISBN 3-900051-07-0.
Raaijmakers, J., Paulitz, T., Steinberg, C., Alabouvette, C., & Moenne-Loccoz, Y.(2009). The rhizosphere: a playground and battlefield for soil borne pathogens and beneficial microorganisms. Plant and Soil, 321, 341–361.
Sanders, P. R., Winter, J. A., Barnason, A. R., Rogers, S. G., & Fraley, R. T. (1987). Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucleic Acid Research, 15, 1543–1558.
Saxena, D., Flores, S., & Stotzky, G. (2002). Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biology & Biochemistry, 34, 133–137.
Scallan, Ú., Liliensiek, A., Clipson, N., & Connolly, J. (2008). RIBOSORT: a program for automated data preparation and exploratory analysis of microbial community fingerprints. Molecular Ecology Resources, 8, 95–98.
Tabari, H., Abghari, H., & Talaee, P. H. (2012). Temporal trends and spatial characteristics of drought and rainfall in arid and semiarid regions of Iran. Hydrological Processes, 22, 3351–3361.
Tapp, H., & Stotzky, G. (1998). Persistence of the insecticidal toxin from Bacillus thuringiensis subsp. kurstaki in soil. Soil Biology & Biochemistry, 30, 471–476.
Tesfaye, M., Temple, S. J., Allan, D. L., Vancze, C. P., & Samac, D. A. (2001). Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum. Plant Physiology,127, 1836–1844.
Tuteja, N. (2007). Abscisic acid and abiotic stress signaling. Plant Signaling & Behavior, 2, 135–138.
Vanjildorj, E., Bae, T. W., Riu, K. Z., Kim, S. Y., & Lee, H. Y. (2005). Overexpression of Arabidopsis ABF3 gene enhances tolerance to drought and cold in transgenic lettuce (Lactuca sativa). Plant Cell, Tissue and Organ Culture, 83, 41–50.
White, T. J., Bruns, T., Lee, S., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. Innis, D. Gelfand, J. Sninsky, & T. White (Eds.), PCR protocols: a guide to methods and applications (pp. 315–322). New York: Academic Press Inc.
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